Rohypnol is a benzodiazepine medication that is medically prohibited in the United States but is lawfully prescribed in more than sixty other countries. The drug is used as a pre-anesthetic and to treat insomnia, but it causes Substantia Nigra function to be lost, which is what motivated this study to determine whether vitamin C and E can improve the effects of adult wistar rats' exposure to Substantia Nigra. Twenty-five adult male Wistar rats were randomly separated into five groups of five rats each (A, B, C, D, and E). Group A, the control group received distilled water. Groups B, C and D received 1mg/kg of Rohypnol, 1mg/kg of Rohypnol+100mg/kg of vitamin C, 1mg/kg of Rohypnol+100mg/kg of vitamin E and 1mg/kg of Rohypnol+100mg/kg of vitamin C and E of Rohypnol respectively. The animals were sacrificed after three weeks of administration and the substantia Nigra were harvested and fixed in 10% formal saline for histological processing and studies. The results obtained in this study following the administration of Vitamin C & E on substantia Nigra exposed rohypnol shows a significant increase in the test group when comparing to the other group. The crucial role of Vitamin C and E in neuronal maturation and functions, neurotransmitter action as well as responses to oxidative stress is well supported by the evidences presented in this study. In conclusion This study have shown that good antioxidants such as vitamin C and E are very much effective in reducing oxidative stress and thus, have great antioxidant ameliorative properties to the Rophynol induced substantia nigra of the wistar rats.