Background: Acrylic resin is the most common material used as a denture base that contacts directly with the oral mucosa. Acrylic resin has a porous surface and surface roughness that allow the attachment and formation of Candida albicans colonies that trigger denture stomatitis. Bilimbi (Averrhoa bilimbi L.) juice contains saponins, phenols, tannins, alkaloids, flavonoids, and triterpenoids, which have antifungal activity against Candida albicans. Purpose: To determine the effectiveness of bilimbi juice on the growth of Candida albicans on heat-cured and cold-cured acrylic resin. Material and method: Experimental laboratory research with a post-test only control group design. 24 heat-cured and cold-cured acrylic resin samples, sizes 10x10x2 mm were divided into 4 groups. Groups I and II were heat-cured and cold-cured acrylic resins immersed in 100% bilimbi juice. Groups III and IV were heat-cured and cold-cured acrylic resins immersed in Aqua®. Each group was immersed for 48 hours, and then the number of colonies was calculated. Results: There was a significant difference in the amount of Candida albicans in heat-cured and cold-cured acrylic resins immersed in 100% bilimbi juice compared with Aqua® (p < 0.05). Conclusion: Bilimbi juice is effective in decreasing the growth of Candida albicans on heat-cured and cold-cured acrylic resins.