The present study is focused on the antidepression and antioxidant activity of the methanolic extract of Trachyspermum ammi. An attempt was made to screen the anti-depression activity of Trachyspermum ammi using in vivo models like the Forced Swim Test (FST), Tail suspension test (TST), and Reserpine-induced depression model using Fluoxetine as standard. In this study both Fluoxetine and META at the dose of 200 mg/kg bd. wt (p<0.0001) and 400 mg/kg bd. wt (p<0.0001) has shown a significant reduction in the duration of Immobility time in both FST and TST when compared to the control group. Reserpine-induced depression model mice were injected with Reserpine (0.2mg/kg bd. wt) prolongation of immobility time in FST was observed. META at the dose of 200 mg/kg bd. wt (p<0.0001) and 400 mg/kg bd. wt (p<0.0001) has shown a significant reduction in the duration of Immobility time FST when compared to disease control. The extract was also evaluated for its antioxidant activity using a Hydroxyl radicle scavenging assay and hydrogen peroxide radicle scavenging assay. From the above, the methanolic extract of leaves of Trachyspermum ammi possessed significant antidepressant and antioxidant activity.