An experiment entitled “Effect of Planting Dates on Growth and Yield of Sugarcane in Subtropical Region of Nepal” was conducted during 2021-2022 at the National Sugarcane Research Program, Jeetpur, Bara, Nepal. The objective of the experiment was to quantify the effect of different sowing time on the growth and yield of sugarcane genotypes. To conduct experiment, the split-plot design was used with two sugarcane genotypes namely Co-0118 and Co-86032 as main plots and six sowing dates (from mid-November to mid-April in an interval of one month) as sub-plots. The results revealed that genotype Co-86032 consistently outperformed Co-0118 in terms of cane yield. The cane yield of Co-86032 was 74.04 t/ha and 104.61 t/ha in 2021 and 2022, respectively whereas the cane yield of Co-0118 was 55.59 t/ha and 86.69 t/ha. Among the sowing dates, mid-November planting produced the highest yields, with Co-86032 yielding 116.63 t/ha and Co-0118 yielding 88.29 t/ha, followed by mid-February planting, which yielded 109.82 t/ha and 84.01 t/ha, respectively. Delayed planting beyond mid-February significantly reduced yields, with Mid-April planting producing only 68.85 t/ha for Co-86032 and 54.35 t/ha for Co-0118 in 2021. This study reflects that mid-November planting was found appropriate for higher cane yield irrespective of sugarcane cane genotypes.