The research goal is to investigate the kinetic coefficients of Tripikon-S. The experiment was conducted on a Tripikon-S reactor scale bench laboratory. Domestic wastewater and anaerobic seed sludge were collected from the WWTP Taman Tun Dr. Ismail in Kuala Lumpur, Malaysia. The kinetic investigation was carried out by continuously pushing the wastewater at five different flow rates into the reactor with a peristaltic pump. The experiment samples of treating wastewater were collected from the reactor's inner and outlet lines to monitor chemical oxygen demand (C.O.D), pH, and temperature twice daily. The activated sludge samples inside the reactor were collected using the specific hose to measure the concentration of Volatile Suspended Solids (V.S.S) three times per week. Ammonia (NH3-N), Orthophosphate (PO4-P), Total Volatile Acids (T.V.A), and alkalinity levels were measured once a week. The Monod formula was used to determine the kinetic coefficients, resulting in the values of the maximum specific growth rate (µmax) of 0.0021 d-1, specific decay rate (Kd) of 0.001 d-1, saturation constant (Ks) of 52.16 mg/L C.O.D., growth yield (Y) 0.062 (g V.S.S)/(g C.O.D) and maximum substrate utilization rate (k) 0.034 (g C.O.D)/(g V.S.S.d) with the treatment efficiency was 97% for COD elimination.