The undertaken study describes to Green-synthesis of iron Nano particles by Ferrous Sulfate Hepta-hydrate (FeSO4.7H20) and utilizing the bio components of root extract of Ipomea. Root extract performed as equally reducing and stabilizing agent. The advancement of green processes for the synthesis of NP is unfolding into decisive derivative of nanotechnology. It has various returns such as, ease with which the method can be maximize, effortless and cost effective etc. To identify the compounds dependable for reduction of Iron ions, the functional groups there in Root extract were scrutinized by Fourier Transform Infrared (FTIR) spectroscopy. The Fe Nano-crystallites of middling size particle have been synthesized by rapid, simple and eco-friendly approach. Iron Nano particles were characterized using Scanning Electron Microscopy (SEM) and EDX. The particles obtained in an agglomerated form. The Antibacterial activity showed no significant effect of the FeNP’s.